cDNA Microarray Analysis on Peach Fruit 2. Preparation of an Array Containing 2,000 Clones.

Abstract

Two cDNA libraries were constructed from peach fruit: young / suitable for harvest. A cDNA microarray was made using about 2,000 clones randomly picked up. Target mRNAs were purified from fruits of 10 , 30, 60, 80, 100 and 120 d after blooming as well as leaves just after flowering time. Using these system, about one tenth of cDNA clones varying expression levels were identified. i) weak in midstage: protein E6 homolog, IAA aminoacid hydroxylase etc., ii) strong in midstage: cinnamoyl-CoA reductase, auxin repressed protein etc., iii) downregulating with maturation: xyloglucan endotransglycosylase, His-containing phosphotransfer protein etc. Grouping of cDNA clones with expression patters will be discussed.