Abstract
Shinji Mizuno, Yosuke Hirasawa, Hiroki Nakagawa, Takahide Sato
The promoter region of CMe-ACS2, a melon ACC synthase gene, contained DRE/CRT cis-acting element(GCCGAC) that was involved in stress-responsive gene expression. By using yeast one-hybrid screening system, we isolated three cDNAs encoding DREB/ERF transcription factors. We named them CMe-DREB1, CMe-ERF1 and CMe-ERF2, respectively.
We confirmed that these transcription factors bound to the cis-acting element by Gel mobilty shift assay and quantified their binding ability by the yeast one-hybrid reporter assay. Furtheremore, we performed transient expression assay in melon leaf by using particul bombardment and measured their transactivation efficiencies. Northan blot analysis showed that the level of CMe-DREB1 transcript was increased by wound and jasmonate treatment, CMe-ERF1 expression was upregulated by ethylene, and CMe-ERF2 expression was increased by high salt stress and cycloheximide, that is protein synthesis inhibitor.
We discuss whether these transcription factors are playing roles in regulating CMe-ACS2 gene expression.
