Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 45
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An Arabidopsis mutant that abnormally accumulates the precursors of seed storage proteins
*Lixin LiTomoo ShimadaYoichiro FukaoMaki KondoMikio NishimuraIkuko Hara-Nishimura
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Pages 774

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Abstract
Seed storage proteins are synthesized on rER as larger precursors, are transported to protein storage vacuoles (PSVs) in receptor-dependent manner, and then are processed into mature forms. Storage protein precursors are accumulated in the extracellular space of Arabidopsis seeds that has a defect in AtVSR1, a vacuolar-sorting receptor. We report here A02 mutant that accumulates the precursors in addition to the mature forms of the storage proteins. Electron microscopic analysis revealed that unlike AtVSR1-deficient mutant, A02 mutant did not secrete the storage proteins. In the cytoplasm of the mutant, there accumulated a lot of electron-dense structures (-1 μm) that are quite different from PSVs. Interestingly, some of the structures were surrounded with ribosomes and thus were assumed to be directly derived from rER. Immunogold analysis indicated that the storage proteins were not distributed in the electron-dense structure but were localized in the periphery or electron-lucent parts of the structures.
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© 2004 by The Japanese Society of Plant Physiologists
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