Abstract
We have previously shown that the cortex of cut hypocotyls completely unite in 7 days, and that leaf gibberellin (GA) is required for this process in cucumber and tomato seedlings. To better understand how the tissue-reunion takes place, histochemical and gene expression analyses were performed using Arabidopsis cut flowering stem. The lowest internode of the main flowering stem was cut through half of its diameter with a micro-surgical knife, and gene expression during the reunion process was analyzed with full-genome microarray. Our microarray analyses of the tissue-reunion showed differential gene expression between cut and non-cut flowering stems, and changes in gene expression during reunion process. The analysis also showed that a number of genes involved in cell division and cell wall modification were up-regulated during the reunion process, and a subset of these up-regulated genes were previously determined to be GA- responsive.
