Abstract
In plants, primary enzyme of carbon fixation, RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase), is encoded as two subunits, rbcL and rbcS . It is reported that the ML tree based on amino-acid sequence of RbcL deduced from the nucleotid sequence alignment makes two clusters; R-type (red linage) cluster and G-type (green linage) cluster. G-type RuBisCO operon genes constitute rbcL-rbcS operon, while R-type RuBisCO genes rbcL-rbcS-cfxQ operon. The cfxQ is predicted to be necessary for the expression of RuBisCO.
cDNA analysis and complete genome sequence of Cyanidioschyzon merolae 10D indicated that cfxQ is coded both in the plastid genome and an the cell nucleus. In this study, we purpose to clear function of two cfxQ , respectively.
Northern hybridyzation analyses of the two genes show the timing of expression of plastid cfxQ is different from that in the cell nucleus.
