Abstract
Although auxin has been considered to regulate many processes in plant development and has been studied for over a centry, the biosynthesis of auxin has remained elusive. Recently it has been reported that YUCCA catalyzes a key step of auxin biosynthesis through the analysis of Arabidopsis mutant, yucca, which overproduces the auxin.
To the auxin biosynthesis and biological function in rice, we isolated OsYUCCA-like1 gene and analyzed its expression pattern in rice, we also produced the overproducer of the OsYUCCA-like1 gene. The plants showed the formation of excess root hairs and abnomal gravitropism, which are similar phenotype to the Arabidopsis yucca mutant.
Increased expression of DR5-GUS in the overproducers also indicated the elevated level of auxin.
Based on these results, we concluded that the OsYUCCA-like1 gene is involved in the step of auxin
biosynthesis pathway in rice.
