Abstract
We established a system in which anthocyanin synthesis of regenerated torenia shoots, which derived from the leaf discs in vitro, was induced on the medium containing high concentration of sucrose. In this system, ABA could also induce anthocyanin synthesis without adding the high concentration of sucrose. Determination of endogenous ABA in the regenerated shoots producing anthocyanin in the presence of high concentration of sucrose revealed that the amount of endogenous ABA transiently increased just before the induction of anthocyanin synthesis. These results suggested that change in the amounts of endogenous ABA might play an important role in the induction of anthocyanin synthesis in the regenerated torenia shoots. In the previous study, we performed cDNA subtraction to isolate the genes up-regulated in torenia regenerated shoots producing anthocyanin. In this study, gene expression profiles of 1000 clones obtained by cDNA subtraction were analyzed by cDNA microarray.
