Abstract
Molecualar mapping of a gene (Als1) responsible for Al resistance in rice was conducted by using a rice mutant(als1) sensitive to Al. F2 seeds were derived from a cross between the mutant and Kasalath. Bulk segregant analysis was performed by pooling equal amounts of DNA from 10 Al-sensitive or 10 Al-resistant F2 plants. A total of 59 InDel markers were selected to examine polymorphism among the Al-sensitive and Al-resistant bulk, the mutant, and the Kasalath. As a result, the marker R6M44 on the long arm of chromosome 6 was linked to this gene. Co-segregating analysis using the 27 Al-sensitive F2 plants showed that R6M44 had a distance of 24.7 cM from (Als1). To further map this gene, two polymorphic InDel markers MaOs0615 and MaOs0619 were developed. Linkage analysis showed that (Als1) was located between MaOs0615 and MaOs0619, with a distance of 3.8 cM and 1.9 cM, respectively.
