Abstract
Generally, the peroxidase(PO) activity in higher plants is known to be upregulated by environmental stresses. PO proteins are encoded by a multigene family, and most of physiological functions of PO isozymes have yet been unclear. In this study, we analyzed the expression of PO genes of poplar as a model tree. ESTs generated from the stress-treated leaves of Populus nigra were subjected to the screening of poplar PO clones, and 198 PO candidates were isolated. Of the candidates clones many were found to be 3'-UTR splicing variants. These PO genes were also confirmed stress-inducible by northern analysis. We are also isolating PO cDNA from the leaf, shoot and xylem of P. alba grown under natural conditions. The stress-induced expression and organ-specific expression of these PO genes will also be discussed.
