Abstract
We previously identified a specific gene encoding a WRKY-type protein, that was immediate-early and transiently activated upon mechanical wounding in tobacco, and designated as Wizz (wound-induced leucine-zipper zinc-finger). In order to examine expression pattern during wound-response, we generated transgenic tobacco plants with Wizz promoter::LUC and 35S::LUC. Results indicated dissected promoter as short as -270::LUC to possess transcription activity. Consequently, 4 types of transfomants with further deleted constructs, -217::LUC,-155::LUC,-97::LUC, and -TATA::LUC were generated and their activities are under examination. The second project is to identify genes that are regulated by WIZZ. To this end, We constructed overexpression transgenic lines and, using its cDNA together with that from wild-type control, We screened a microarray, containing approximately 4,000 genes originated from N. sylvestris and 48 wound-response genes from N. tabacum. A preliminary hybridization showed a considerable number of genes to be differentially expressed between the two, and their identity is now under progress.
