Abstract
Gene targeting by homologous recombination (HR) is a powerful tool for reverse genetics. We have developed a gene-targeting system in rice based on large-scale transformation with strong positive-negative selection and succeeded in modifying the Waxy gene. To access the applicability of our targeting system, we are trying to knockout the Alcohol dehydrogenase genes, Adh1 and Adh2, which are arranged as direct repeats separated by 30 kb on chromosome 11. Previously, we reported our attempt of targeted modification of both Adh1 and Adh2 separately and results including Southern analysis of the targeted T0 plants (2004). Here we report the analysis of T1 plants having Adh2 targeted as well as phenotypic characterization of Adh1 modified plants. Based on these results, we will discuss the implications of HR in somatic rice cell, which is involved in T-DNA mediated foreign genes integration.
