Transcriptome and metabolome analyses to estimate differences between pre- and post-cryopreserved cells of Arabidopsis thaliana

Abstract

In the previous annual meeting, we reported a newly developed protocol for cryopreservation of Arabidopsis suspension-cultured cells. In this study, we evaluated how the preservation affected gene expression and metabolite production by transcriptome and metabolome analyses. Independent three experiments were carried out for the evaluation. After recovering the cells from liquid nitrogen, the transcripts were analyzed using the Agilent 22K microarrays. The transcripts of 46 genes that exhibited more than 2 fold changes were reproducibly detected among the triplicates, while duplicated self hybridization detected only 4 genes, indicating that the cryopreservation protocol affected gene expression pattern of the genes. Gas chromatography mass spectrometry analyses of metabolites of the cell lines also indicated some changes in metabolites. The results provided us fundamental information of the effects of the cryopreservation protocol.