Abstract
In order to study the physiological role of purple acid phosphatase in tobacco cells (NtPAP12), NtPAP12 was constitutively over-expressed in cultured tobacco cells. The expression of NtPAP12 resulted in the promotion of cell growth. At the early stage of wall regeneration in tobacco protoplasts, the expression of NtPAP12 accelerated the deposition of β-glucan, which was observed using Calcofluor staing. The enhancement of the deposition was visualized after 15 min and reached maximum at 60 min. Based on methylation analysis, tobacco protoplasts produced cellulose as a major component on the surface of plasma membrane. These results showed that the expression of NtPAP12 enhanced the cellulose deposition on the surface of plasma membrane.
