Abstract
In plant, as in mammalian tissue, exocytotic and endocytotic events have been identified with stepwise changes in capacitance. We aimed to find the SNARE protein the appeared to the nodule formation, and to analyze the function of the proteins. We used Lotus japonicus and found one gene (Sn6) that express at the high level in nodules. As a result of in situ hybridization, the signals were appeared at the top of nodule primodia when the nodule grew up, strong signal was appeared in the cortex of surrounding the nodules. To prove that Sn6 gene was required for the division and extension of the plant, we cloned the antisense or sense Sn6 gene. As a result, there were about 60-70% weight of the T1 seeds of the antisense Sn6 construct. The growth rate of the antisense Sn6 construct were slowly compared with non-transformant. Now I am analyzing in more details.
