Formation of a novel thiadiazoline derivative in plants in response to fumigation with reactive nitrogen species

Abstract

We have previously reported that about one-third of the total nitrogen derived from NO₂ and nitrate taken up by plants including Arabidopsis thaliana was converted neither inorganic nor Kjeldahl nitrogen (Morikawa et al., 2004). We refer this nitrogen as unidentified nitrogen (UN). More recently, we have identified a derivative of thiadiazoline (Δ²-1,2,3-thiadiazoline) as a UN-bearing compound (Miyawaki et al., 2004). This thiadiazoline derivative had a five-membered hetero ring consisting of one sulfur, two nitrogens and two carbons. A literature search including Scifinder revealed that there is very little description of Δ²-1,2,3-thiadiazoline. We are currently investigating quantitation and biosynthesis of this thiadiazoline derivative in plant leaves in response to various stresses.
H. Morikawa et al., (2004) Planta 219:14-22
K. Miyawaki et al., (2004) Org. Biomol. Chem. 2: 2870-2873