Abstract
Arabidopsis ABI3 and the homologous genes are known as transcriptional factor which expresses specifically in embryo from very early stage of embryogenesis. In our previous work, we had identified an embryonic promoter cis-element, Carrot Embryonic Element 1 (CEE1), in the C-ABI3 (carrot-ABI3) promoter. And we had shown that 150 bp fragment of ABI3 promoter region (Atd3-1L1) including two reverse-CEE1-like sequences (AEE1-1 and AEE1-2) is related to the ABI3 gene expression in embryo. In this study, we analyzed precisely the Atd3-1L1 region related to the embryonic expression. The result indicated that AEE1-1 sequence is important for gene expression in embryo. On the other hands, OsEE1 sequence which was found in OsVP1 promoter can bind with carrot CEE1-binding-factors. CEE1, AEE1-1 and OsEE1 are very similar sequence, which might be related to gene expression in embryos of various plants.
