Abstract
Photosystem (PS) II is surrounded by its light-harvesting complex II (LHCII) where more than 30 proteins in total are postulated to be involved. LHCII proteins are currenlty thought to reversibly migrate from PSII to PSI in response to the redox state of plastoquinone pool (state transition). Thus the size of PSII-LHCII supercomplex is variable depending upon the environmental light: more LHCII could be attached to PSII in state I, and to PSI in state II. To determine the precise mechanism of photoacclimation of photosystems including state transition, isolation of the holo complexes are demanded. In this study, we report purification of the PSII-LHCII supercomplex from the thylakoid membrane of Chlamydomonas reinhardtii strain harboring His-tagged CP47 by nickel column chromatography.
