Abstract
The cyanobacterial Rbp proteins are RNA-binding protein with a single RNA recognition motif (RRM). The unicellular cyanobacteria Synechococcus sp. strains PCC6301 and PCC7942 have three homologous genes, rbp1, rbp2 and rbp3, encoding Rbp1 (107 aa), Rbp2 (99 aa) and Rbp3 (142 aa). In our previous research, we reported that the expression of rbp1 was cold-inducible and the rbp1 disruptant grew poorly at low temperatures, whereas that of rbp2 was cold-depressed and the rbp2 disruptant grew normally even at low temperatures. In order to characterize three rbp promoter activities under the different stress conditions, chimeric genes containing each rbp upstream sequence fused to the bacterial luciferase (luxAB) as a reporter were transformed in Synechococcus PCC7942. We report the results of the promoter responses of three rbp genes under the different stress conditions.
