Abstract
Tm-1 is a semi-dominant trait in tomato that inhibits the multiplication of Tomato mosaic virus (ToMV) within a single cell without inducing the hypersensitive cell death. Tm-1 has been widely introduced in commercial tomatoes, however, it remains unidentified because of its difficulty in genetic mapping. Recently, an in vitro translation-replication system for ToMV RNA has been established using the lysate of evacuolated protoplasts from uninfected tobacco BY-2 cells. In the BY-2 lysate supplemented with the Tm-1 lysate, ToMV RNA replication was specifically inhibited without any detectable effect on the accumulation of ToMV replication proteins. This result suggests that the Tm-1 factor inhibits the function of ToMV replication proteins, but does not affect the production or the stability of these proteins. The property of the Tm-1 activity on the liquid chromatography will also be reported.
