Abstract
LSD1 (Lesion Simulating Disease 1) is a negative regulator of oxidative stress-induced cell death involved in disease resistance. To clarify the function of LSD1 as a regulatory protein, we are focusing on the protein-protein interaction partners identified by yeast two-hybrid screening. One of those, AtbZIP10 specifically interacts with LSD1. Although AtbZIP10 has a potential NLS, these are co-localized in cytoplasm. However any change of AtbZIP10 distribution was not observed in lsd1. Using split YFP analyses, we could confirm the LSD1-AtbZIP10 interaction in planta. The abolishment of AtbZIP10 partially suppressed the lsd1-induced runaway cell death (lsd1-rcd). On the other hand, AtbZIP10 overexpression induced more lsd1-rcd. Similarly AtbZIP10 overexpressor demonstrated more resistance to both compatible and incompatible Peronospora Parasatica (P.p.), whereas enhanced susceptibility to incompatible P.p. was observed in atbzip10. Taken together, AtbZIP10 would regulate the target genes involved in the programmed cell death at the hypersensitive response in plant disease resistance.
