Abstract
Pseudomonas cichoii (Pc) is the causual agent of bacterial rot of lettuce. The disease development required de novo protein synthesis of lettuce cells. Cell death was induced at 9 to 12 hour after inoculation with Pc. Heterochromatin condensation and DNA fragmentation were also observed. From the results of pharmachological experiments, induction of the cell death was thought to mediated by intracellular reactive oxygen species, protein kinase, protease and DNase.The genes specifically regulated by Pc were isolated by differential display.Translation initiation factor, hsp70, protein kinase and chitinase were regurated by Pc inoculation.These results suggested that apoptotic cell death might have crucial role in the development of bacterial rot disease caused by Pc.
