Abstract
Several environmental stress induces seeds sterility in a lot of plant species via injury to their reproductive development. However these molecular mechanisms are not well understood. We study the high-temperature injury of barley microsporogenesis at the molecular level. Barley is a useful experimental plant for investigation reproductive development, because its development is well synchronized. High-temperature stress (30oC day/25oC night) during early development of anther cell layers causes complete male sterility. In this presentation, we would like to show the result of differences of entire protein expressions between high-temperature injured panicles and control panicles. We found several proteins fluctuated by high temperature stress via proteomics analysis including 2D SDS-PAGE and peptide mass finger printing wiht MALDI-TOF MS.
