Abstract
We are in the process of several large-scale sequence analyses aimed
at elucidating the whole genetic system involved in nitrogen fixation
and photosynthesis. One of such projects is the genome analysis of
the model leguminous plant, Lotus japonicus. Using the
information on ESTs and cDNA markers of legume plants, genomic clones
corresponding to the multiple seed points of the genome have been
chosen as initial targets. As the accumulation of the seed sequences
has progressed, clone selection by walking has also initiated. To
analyze long-range genomic sequence rapidly, we developed an
annotation pipeline to automate gene-finding. The sequence data,
annotation and mapping information are available through the WWW at
http://www.kazusa.or.jp/lotus/. In this presentation, we will review
on the current status of our intensive genomic studies including
generation of DNA markers and a genetic linkage map, construction of
genome and cDNA libraries and their sequencing of Lotus
japonicus genome.
