Abstract
A chloroplast is the most important organella in plant involved in photosynthesis and production of various metabolites, but the most proteins are the nuclear-encoded. In Arabidopsis, a large-scale analysis of mutants is possible because many tag lines exist. For the functional analysis of the nuclear-encoded chloroplast proteins, we systematically collected these tag lines. 2,090 chloroplast proteins are identified by at least 3 of the 4 predictors (iPSORT, PCLR, Predotar, and TargetP), and 1,277 genes were disrupted by insertion of Ds or T-DNA, which is about 61 % of 2,090 chloroplast proteins. This collection of tag lines become larger as numbers of tag lines increase. We have not only isolated albino or pale-green mutants (apg), but also collected homo lines without clear phenotypes when plated on agar medium. Using these homo lines, various screening will be performed to identify novel functions of chloroplast protein.
