Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 46
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"A" rich sequences in immediate before translation initiation codons
*Tsuyoshi ImaiToshiya YamamotoTateki HayashiJun-ichi Soejima
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Pages 461

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Abstract
The sequences around translation initiation codons (from -20 to +13) were compared among 271 cDNA clones obtained from peach fruit. Following features were found: 1) three or more consecutive bases containing single kind or repetitive sequences which is made up of six or more bases are frequently existed in 5' untranslated regions. 2) from -4 to -1, "A" appears frequently than others, namely 43% at -1 and "AA" at -2 to -1 is 25%. 3) "G" appears 72% frequency at +4, "C" at +5 is 55%. Accordingly, most frequently found amino acid residue at second is Alanine with a 40% frequency. 4) in frame stop codon is found in 49 clones (18%) and out of frame in 109 clones (40%). Collectively, many different features are found in the sequences before and after the initiation codons, which may be correlated with establishment of translation initiation point or with efficiency of translation.
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© 2005 by The Japanese Society of Plant Physiologists
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