Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 46
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Cluster Analysis of the Gene Expression Data by the Oligoarray System in Callus Formation or Regeneration Process of Rice
*Koji DoiKouji SatohShigemi IizumiSetsuko KimuraHisako OokaKimihisa TasakiHitomi Akiyama-YamadaJung-Sook LeeShoshi Kikuchi
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Pages 468

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Abstract
We are running various experiments using the rice 22K oligoarray based on the sequences of full-length cDNA clones. In this presentation, we will discuss about a preliminary cluster analysis performed to understand the relationship between gene expression data in callus formation or regeneration process.
Thirty-four data set of microarray experiments were combined, and K-means clustering was performed based on similarity of the expression pattern obtained from these experiments. Clones were classified into 100 clusters by this clustering. The smallest cluster contained 5 clones, while the largest cluster contained 384 clusters.
The result was stored in a newly constructed relational database, which also includes various annotation information from rice full-length cDNA database (KOME), and Gene Ontology datasets, which is an standard reference in integration of gene annotation data. Based on this database, we surveyed the possibility of the genes as contributors of callus formation or regeneration process.
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© 2005 by The Japanese Society of Plant Physiologists
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