Abstract
Under phosphate deficiency, phosphate re-translocation from older organs to younger organs is one of the important adaptation mechanisms. In the present study, to understand the molecular mechanisms of phosphate re-translocation, we measured phosphate distribution in shoot and searched potential genes involving phosphate re-translocation in Arabidopsis.
First, we measured content of phosphate in each leaf under various phosphate levels in culture media. Second, re-translocation of 32P was observed by an imaging plate system. During these studies, plants were cultured in a newly developed hydroponic culture system. We determined source and sink leaves based on the distribution analysis. Then, we performed microarray analysis of m-RNA isolated from source and sink leaves. We discuss the possible roles of genes which changed transcriptional levels between source and sink leaves in phosphate re-translocation.
