Abstract
We have reported in the previous meeting that the application of recombinant precursor proteins on protein import into chloroplasts. Currently, we are developing the methods to visualize protein import into chloroplasts in vitro. We have obtained the encouraging results if recombinant precursor proteins were modified with fluorescent compounds. In brief, after import reaction, chloroplasts are solubilized with the SDS sample buffer, followed by the SDS-PAGE. The gel in wet condition was directly loaded on the fluorescent imaging-analyzer and scanned. In this way, the processing of recombinant protein, resulted by the precursor to be imported into chloroplasts, was visualized. The rate of import was also able to be analyzed. We will present these results and our current efforts at the meeting.
