Abstract
SNAREs (*Soluble N-ethyl-maleimide sensitive factor attachment protein receptors*) play a critical role in membrane fusion process of vesicular transport system. Genome studies on Arabisopsis thaliana pointed out that there are 54 genes (much more than yeast and mammalian) which encode SNARE proteins. The abundance of SNARE genes indicates the complexity of endomembrane trafficking within the plant cell. SYP7-group containing three genes, SYP71, SYP72 and SYP73, has no homologues in mammalians and yeast, suggesting that SYP7 group has plant unique functions. We already reported that SYP71/72/73 (SYP7-group) was localized on the ER by transient expression system. However, localization studies of GFP-fused proteins by*using BY2 Cell, showed that the proteins were not only localized on the ER, but also on the plasma membrane. Furthermore, we are analyzing membrane trafficking in plant involving SYP-7s proteins by generating transgenic plant expressing GFP-fused SYP7-group proteins and performing promoter analysis by using GUS reporter.
