Abstract
Clathrin has been suggested to be involved in removal of an excessive membrane in cell plate formation. In this study, we analyzed the role of clathrin in the formation of cell plate using tobacco cultured cell, BY-2. Immunocytochemical study using an antibody against clathrin revealed that this protein was distributed in cell plate. To further analysis the role of clathrin in cell plate formation, C-terminus of clathrin heavy chain, in which light chain binding domain and trimeraization domain are contained, was expressed in BY-2 cells. When the cell cycle of the transformant was syncronized, about half of the cell became multinucleate, and morphology and organization of cell plate and microtuble bundles in phragmoplast were aberrant.This result suggested that clathrin is involved in the formation of cell plate.
