Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 46
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Search for novel calcium channel blockers and their effects against TPC1 type channel
Cun LinTakashi KadonoTakuya FuruichiKazuharu Yoshizuka*Tomonori Kawano
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CONFERENCE PROCEEDINGS FREE ACCESS

Pages 534

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Abstract
Effects of rare earth elements (atomic numbers, 39, 57-60, 62-71; Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu) and Al added as chloride salts, on Ca2+ influx induced by two inducers of Ca2+ influx via distinct calcium channels, namely hypoosmotic shock and salicylic acid, were examined in a suspension-cultured transgenic cell line of BY-2 tobacco (Nicotiana tabacum) cells expressing a Ca2+-sensitive luminescent protein, aequorin. Most metals showed inhibitory effect against Ca2+ influx. Especially Nd, Sm, Eu, Gd and Tb showed the most robust inhibitory action. Interestingly, selective inhibition of the salicylate-induced Ca2+ influx by Pr at a low dose (ca. 0.1 mM) and by Al at high doses (5 - 10 mM) was observed. Lastly, the effects of above putative inhibitors were examined in the cell lines with modified TPC1 type channel expression.
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© 2005 by The Japanese Society of Plant Physiologists
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