Abstract
The most major prevalent lesions produced by ultraviolet (UV) are cyclobutane (CPD) and pyrimidine (6-4) pyrimidone dimmers. These photoproducts impair DNA replication and transcription processes and induce mutation. In plants, these photoproducts are mainly restored with photolyase and NER. Moreover, it has been thought that these lesions which are not restored by photolyase and NER should be restored by homologous recombination (HR).
In the present study, we have analyzed intrachromosomal homologous recombination (ICHR) frequency in AtRAD1 or AtRAD2 knock-out mutant of Arabidopsis . With the result, AtRAD1 KO (uvh1 ) suppress ICHR, on the other hand AtRAD2 KO (uvh3 ) enhance ICHR. We have crossed photolyase deficient mutant of Arabidopsis (uvr2-1 ) with uvh1 or uvh3 to produce double KO mutant, uvr2-1 / uvh1 or uvr2-1 / uvh3 , respectively. We are currently analyzing ICHR frequency in these double KO mutants.
