Abstract
Plants are exposed with many environmental stressors that are accompanied with generation of ROS. It is important to know early responses of plants against the oxidative stressor. We found that expression of Glutathione S-transferase 3 (AtGST3) has occurred within 1hr when plants were expose to ozone. Although it has reported that AtGST3 expression is regulated with SA and ethylene, it can't explain early response of AtGST3 because more than several hours were needed to induce AtGST3 expression with treatment of phytohormones. Alternatively, we employed regulation system of GST gene expression in animal as a model for regulation of AtGST3 expression. In the model, Keap1-Nrf2 system regulates GST expression through a cis-element, designated as antioxidant responsible element (ARE). We found an ARE in AtGST3 promoter region and analyzed its function using GST3::GUS transformants. Moreover, we analyzed AtGST3 expression in knockout mutants of Keap1, MAPKs, and transgenic plants introducing animal Nrf2 gene.
