Abstract
Ascorbic acid (AsA) has the important role of the antioxidant action and the control of the transcriptional induction of the defense gene in the plant. We reported the increase of the AsA on a tomato by JA treatment, and it was suggested that this depends on the increase of the transcription on L-galactono-γ-lactone dehydrogenase (GLDH) in the AsA biosynthesis. It is thought that GLDH was important about the control in the biosynthesis. Thus GLDH antisense tomato was created and the mutants show about 30-90% of activity of the wild strain. AsA quantity of these mutants was low generally compared with the content of the wild strain but isn't always correlated with GLDH activity. Moreover, the ratio of the reduced and the oxidized type didn't show a constant tendency and it suggested the complexity of the controlling mechanism to the AsA accumulation and the anti-oxidant action.
