Abstract
The addition of cAMP resulted in acidification of the suspension medium of a wild-type and flagellate-defective mutant of Chlamydomonas reinhardtii in the dark. These phenomena were pronounced in cells grown with nitrate or glycine as a sole nitrogen source, while these phenomena were not observed in cells grown with ammonia as a nitrogen source. The addition of 3-isobutyl-1-methyl-xanthine, an inhibitor of phosphodiesterase which catalyze the conversion of cAMP to 5`AMP, enhanced the acidification in cells grown in glycine as a sole nitrogen source, while the addition of this inhibitor did not show any effect on pH in cells grown in ammonia as a sole nitrogen source.
The gamete formation in Chlamydomonas reinhardtii occurred in cells grown with ammonia, nitrate and glycine as a sole nitrogen source. The role of blue light and cAMP is discussed in relation to the transport process of Chlamydomonas reinhardtii.
