Abstract
It is important to determine the genes controlling nitrate accumulation in higher plants. We selected and analyzed a low nitrate accumulator mutant of Arabidopsis thaliana (columbia). The candidate of mutant line was selected from 1372 mutant lines of EMS-mutagenized M2 seed progeny by determining leaf nitrate concentration. The χ2 analysis using F2 plants generated by crossing M3 plants and wild type indicated that one gene is related to the mutation. The physiological characteristics were investigated using F3 plant (F3.522a7). Ten seeds of F3.522a7 or wild-type were hydroponically grown for 3-weeks with 5mM nitrate. In the mutant leaf nitrate concentration was about 60% of that of wild types although it was same levels as the wild type in the mutant root. Nitrate reductase activity was similar between the mutant and wild-type. Mapping analysis of the mutant is being undertaken.
