Abstract
The Arabidopsis slr (solitary-root) mutant which has a gain-of-function mutation in IAA14, a member of Aux/IAA family, has no lateral roots (LRs). On the other hand, the ssl2 (suppressor of slr2) is a single recessive mutation which partially suppresses the slr phenotype in LR formation. SSL2 encodes CHR6/PICKLE/GYMNOS, a homolog of the CHD3/Mi-2 in animal SWI2/SNF2 ATPase chromatin remodeling factors. It is known that CHD3 is a subunit of the NuRD complex that mediates transcriptional repression through histone deacetylation and nuclueosome remodeling. Therefore, SSL2/CHR6 is thought to mediate transcriptional repression through chromatin remodeling, but it is unknown how SSL2/CHR6 is involved in auxin-regulated LR formation. In order to identify genes regulated by SSL2/CHR6 in LR formation, we compared gene expression profiles between slr and ssl2 slr double mutant by using Agilent Arabidopsis2 DNA microarray. We will present the data on the expression and functional analyses of several identified gene.
