Abstract
In Arabidopsis thaliana, as much as 10 cyclin D (CYCDs) were identified. One of these cyclin D, CYCD4;2, lacks Rb binding morif and is considered to be an atypical cyclin D. In this research, we performed a functional analysis of CYCD4;2 to elucidate the unknown function of plant cyclin D.
In insect cells, CYCD4;2 could make a complex with CDKA;1, CDKB1;1 and CDKB2;1.We could also detect the interaction between CYCD4;2 and CDKA;1 in plant cells. To study the role of this cyclin during plant development, we generated transgenic plants that constitutively overproduce CYCD4;2. These plants grew normally, but accelerated cell division was observed in a specific cell type. These results suggest that CYCD4;2 makes a complex at least with CDKA;1 and controls cell division in a cell-type specific manner.