Abstract
It is widely believed that chloroplast was generated by endosymbiosis of an ancient photosynthetic bacterium with an ancient eukaryotic cell. arc3 mutant of Arabidopsis thaliana is a chloroplast division mutant. ARC3 gene is a fusion gene of prokaryotic, FtsZ, and eukaryotic, phosphatidylinositol-4-phosphate 5-kinase (PIP5K), genes. ARC3 protein is exclusively located in chloroplast division ring as well as A. thaliana FtsZ. To elucidate the function of ARC3 protein, we analyzed the interaction factor(s) with ARC3 protein by yeast two-hybrid system or ARC3 affinity column. The yeast two-hybrid system revealed that ARC3 could interact with ARC3 itself, and form the polymer. We will report the ARC3 interaction factor(s) in detail.
