Abstract
In higher plants, peroxisomes have essential roles such as fatty acid degradation and photorespiration. To better understand peroxisome biogenesis, we attempted to generate mutants having peroxisomes with different sizes, shapes or subcellular localization. We used seeds of a transgenic Arabidopsis termed GFP-PTS1 in which peroxisomes can be visualized with GFP. GFP-PTS1 seeds were mutagenized chemically and allowed to germinate. Of about 37,000 M2 plants, we identified eighty-two mutants that have abnormal peroxisome morphology based on the pattern of GFP fluorescence. apm (aberrant peroxisome morphology) mutants exhibited the tissue- and/or organ-specific patterns of GFP as follows, (1) long peroxisomes, (2) giant peroxisomes, (3) GFP fluorescence in the cytosol as same as in peroxisomes, and (4) different distribution. We reported that the mutation in DRP3A caused the morphological change to long peroxisomes. In this presentation, we would like to discuss the phenotypes of another apm mutants.
