Abstract
RNA editing alters genomic nucleotide sequences at the transcript level. In tobacco (Nicotiana tabacum) chloroplasts, C-to-U RNA editing has been detected at 34 sites. We identified newly two editing sites from N. tabacum chloroplast transcripts. However, little is known about the mechanism of site-recognition and physiological meanings of RNA editing. Our research group has developed an in vitro RNA editing system from tobacco chloroplasts. Using this system, the cis-elements of four editing sites were identified. For comprehensive analysis of RNA editing, more simple systems are necessary. We successfully developed a convenient in vitro system without using RI from tobacco chloroplasts. Here, we report the results about the in vitro editing efficiency of many editing sites of tobacco chloroplast transcripts by using this system.
