Abstract
F-box proteins play roles in SCF complexes. F-box protein is a one of the component of SCF complex, and act as a receptor for substrate proteins of ubiquitination. By database analysis, we found 568 F-box protein genes in Arabidopsis genome, however, biological functions of most of the genes have not been cleared. We are trying to understand the unknown functions of the F-box proteins. The knowledge of subcellular localization patterns of proteins is helpful to understand biological functions of unknown proteins. To investigate the subcellular localization of F-box proteins we carried out in silicao analysis using PSORT program.This analysis suggested F-box protein are localized in many different intracellular positions. Intestinally 39 and 24 F-box proteins were suggested to be localized in mitochondria and chloroplast, respectively. To study subcellular localization of F-box proteins in vivo we have started the analysis of protein localization using GFP fusion proteins.
