Abstract
Rice MTR1 (methyltryptophan resistant 1) mutant callus line accumulates a large amount of Trp in the tissue. The effects of the mutation on the metabolic profile of rice callus was examined. In addtion to Trp, the level of Phe in the mutant was increased by 20-fold, compared to wild-type (N8) callus. The level of Tyr was not affected by the mutation. The metabolic profiling analysis using HPLC-PDA indicated that, bsides Trp and Phe, MTR1 calli remarkably accumulated several UV-active metabolites. One of these metabolite was identified as feruloylquinate by 1H-NMR and ESI-MS/MS analyses. These results suggested that MTR1 mutation activated the biosynthetic pathway of Phe-related metabolites as well as that of Trp.
