Abstract
In order to get more insight into the ABA signaling pathway, we conducted screens for Arabidopsis mutants that have an altered ABA sensitivity using ABA analogs. Using PBI-51, an ABA analog, we obtained several putative ABA hypersensitive mutants, named ahg (ABA hypersensitive germination). We also searched for ABA analogs that might have ability to allow us to dissect the ABA signaling pathway. We found one analog, named #18, has a growth inhibition effect like ABA but its effect is much stronger on the growth of greening portion than that of root. Based on the response of known ABA-related mutants and several ABA inducible genes to this analog, #18 seems to actually evoke the ABA response. We are trying to identify the loci that affect the sensitivity to this analog by isolating mutants or searching for ecotypes with altered sensitivity to this analog. The effectiveness of analog usage will be discussed.
