Abstract
Most Chl a in the PS1 RC complexes was removed without any loss of P700 by ether treatment, yielding the antenna-depleted PS1 RC complexes that contain 1 Chl a' and 10-14 Chl a per P700. (1) On addition of Chl a' with phospholipids (PG), 5-10 Chl a' per P700 bound to the antenna-depleted complexes. (2) The P700 photooxidation was enhanced in the Chl a'-reconstituted complexes, suggesting that the bound Chl a' acts as efficient antenna as Chl a remaining in the complexes. (3) When Chl a' was reconstituted with the equal amounts of Chl a, the Chl a' was less bound than Chl a, suggesting the lower affinity of Chl a' than Chl a to the Chl a-binding sites. (4) The more extraction of antenna Chl a resulted in the improper binding of Chl a' to the Chl a-binding sites.
