Abstract
The SNF1/AMPK kinases are highly conserved in eukaryotes. Protein kinases of this subfamily exist as catalytically active hetero-trimeric complexes consisting of α, β and γ subunits. Previous reports indicate that interaction between α and γ subunits of SNF1 kinase are strongly in accordance with the deprivation of glucose in the growth medium. Our yeast two-hybrid experiments with the three subunits rice SnRK1 complex convincingly show that the interaction not only between α and γ, but also β and γ subunits is mediated by deprivation of glucose in the growth medium. Some of the experimental evidences also suggest that both dimeric and trimeric forms of protein complexes may exist in vivo .These findings suggest that the regulatory γ subunits of rice SnRK1 may recognize specific signals and interacts with the dimeric complexes comprising of α and β subunits and thus stimulate the catalytic activity of the enzyme complex.
