Abstract
Most of the chloroplast proteins are nuclear-encoded. For the functional analysis of the nuclear-encoded chloroplast proteins in Arabidopsis, we systematically collected these tag-lines. 2,090 chloroplast proteins are identified by typical 4 predictors. Base on database of RIKEN, NASC, Wisconsin, CSHL and SALK, we collected 3,416 tag-lines disrupted by transposon or T-DNA. From this collection, we have not only isolated mutants with visible phenotype (apg mutants), but also collected homo-lines without clear phenotypes when plated on agar medium. We identified 55 mutants showing abnormal seedling by screening of 704 of RIKEN Ds tag-lines. To determine whether these mutations are caused by a Ds insertion, we examined mutant alleles existed in other tag-lines. Among 33 genes, 24 genes existed two or more alleles, and mutant alleles of 17 genes showed similar phenotype. These results suggest that at least 17 genes tagged with Ds and have an important role for chloroplast development.
