Abstract
Using talin actin-binding domain fused to tdTomato, a new RFP variant (Shaner et al. 2004), actin filaments were visualized in both moss and fern cells. We have been studying actin dynamics during photoresponses in GFP-talin-expressing line of Physcomitrella. Blue excitation of GFP, however is problematic because it interferes blue-light responses. Therefore we tried to visualize actin by green-excitable label. The same actin dynamics as in GFP-talin transformant could be observed in tdTomato-talin-expressing line. Further, by transforming tdTomato-talin into the line stably expressing GFP-tubulin, actin filaments and microtubules were observed in the same cell. In fern Adiantum cells, actin filaments had been scarcely observed by expression of GFP-talin. Actin filaments were clearly observed in fern cells by tdTomato-talin expression
