Dynamic rearrangement of actin microfilaments and their roles in cell palte formation during mitosis in synchronized tobacco cells

Abstract

The actin microfilaments (MFs) have essential roles in plant cell division and morphogenesis. However, little is known about the dynamics of the MF structure during cell division. To observe MFs in living plant cell, we have already established a transgenic BY-2 cell line expressing GFP-Fimbrin (BY-GF). Using this cell line, we have found that MF structure at cell cortex in mitosis appeared to form two bands, and named the structure MF twin peaks (MFTP).
In this study, we examined the roles of MFTP in cell plate formation. Disruption of MFTP by pulse-treatment of cytochalasin D induced distorted cell plate, while disruption of actin phragmoplast had little effect on cell plate guidance. These results suggested that MFTP play important roles in cell plate guidance.