Abstract
An approach to understand the function of transcription factors (TFs) that coordinately regulate expression of genes involved in metabolic pathways is important for metabolic engineering in plant. As a basis for the functional analysis of TFs, we have investigated the potential TF genes encoded in the Arabidopsis genome and collected sequence data for 1860 genes encoding known and putative TFs that belong to 56 gene families. For the further functional analysis, ERF, DOF, STK, CO, BRX, NIN and MYB families were selected, and their ORF cDNA were subcloned into the Gateway entry vector. Then, we transformed Arabidopsis cultured cells (line T87) with cDNAs of the selected TF genes driven by a CaMV35S promoter. We examined transcriptional profiles in the TF gene-overexpressed T87 calli using an Arabidopsis DNA microarray. From the results, metabolic pathways controlled by the TF genes are discussed.
